Light up fluorescence, illuminate the road to scientific research ——I wish the Abbkine IF experimental training a successful start

 

On November 5, 2020, under the caring leadership of superior leaders, our company successfully carried out the on-site training of “Immunofluorescence (IF)” for all Yakin employees. This training activity is an innovative activity that integrates theory and practice and conforms to the development trend of biotechnology. It has certain reference significance for the examination of biological professional knowledge in the future. At the same time, it once again embodies the exploration of the true knowledge of scientific research by the Yakein people, and responds to the resounding slogan of “lighting up the fire of fluorescence, illuminating the road to scientific research”.

In order to successfully complete this experimental operation, since its establishment, our company has invested a lot of funds to improve the consumables and equipment of scientific research laboratories. The superior leaders are also particularly concerned about the

Essential guidance 2 | Fishing with Purekine “fishing gear”, Protein Association “wishers hooked”

 

 

In the last issue, the editor brought you “Dry Goods | Using IP “Hook” Fishing, Protein Club “Wishers Take the Bite””, and the response was very enthusiastic. In the voice of everyone, the editor is rushing through the previous experimental notes, bringing you the second bullet of protein interaction knowledge-Pull-Down. If the protein interaction is compared to an interesting “fishing” activity, then the Purekine filler we use is a tough and slender “fishing rod” and “fishing line”. The known GST-labeled recombinant protein is sharp The strong “fish hook” and “fish bait” are the perfect combination, and our target protein is the “fish” that moves freely in the water.

The concept of Pull-Down is similar to Co-IP, and its purpose is to study proteins or ligands that bind to known bait proteins. Pull-Down aims to prove the interaction between t

There is finally a cure for neurodegeneration!

Professor Hilmar Bading and his team’s research focused on NMDA receptors. This receptor is an ion channel protein, activated by the neurotransmitter glutamate, allowing calcium to flow into the cell. The calcium signal plays a role in the plasticity of synapses, but it also spreads to the nucleus, where it activates a protective genetic program. The NMDA receptors activated by glutamate are located at the junctions of nerve cells and play a key role in the brain, contributing to the learning and memory process and neuroprotection. But the same receptors also exist outside the synapse. These additional synaptic NMDA receptors pose a threat because their activation can cause cell death. However, under normal circumstances, an effective cellular uptake system of glutamate ensures that these receptors are not activated, and nerve cells therefore remain intact.

But when diseases occur, for example, if certain parts of the brain cannot get enough oxygen after a stroke, the i

Using immunoprecipitation “fish hooks” for fishing, the protein  will always “hooked with willing”

 

The thoughtful Thursday is here again, and the editor who has long been unable to bear it is finally released to meet our favorite “Yin Fans”.

This time, the editor brings you a wave of colorful biological science mysteries-protein interaction. If protein interaction is compared to an interesting “fishing” activity, then the tag antibody coupling carrier we use is a sharp and strong “fish hook”, and the tag recombinant protein (bait protein) is sweet and delicious. “Bait”, and our target protein is the “fish” that moves freely in the water.

If you want to do your job well, you must first sharpen your tools. Maybe you are still worrying about the purification and enrichment of the target protein without a good antibody coupled to Beads. Maybe the protein content you collected at this time is low, maybe you are enriching it at this time. The protein impurity content is high, maybe the protein vital

A Common test of Hydrogen Peroxide

 

Hydrogen Peroxide is a peroxide and oxidizing agent with disinfectant, antiviral and anti-bacterial activities. Upon rinsing and gargling or topical application, hydrogen peroxide exerts its oxidizing activity and produces free radicals which leads to oxidative damage to proteins and membrane lipids. This may inactivate and destroy pathogens and may prevent spreading of infection.

 

Hydrogen peroxide is unstable and slowly decomposes in the presence of light. Because of its instability, hydrogen peroxide is typically stored with a stabilizer in a weakly acidic solution in a dark coloured bottle. Hydrogen peroxide is found in biological systems including the human body. Enzymes that use or decompose hydrogen peroxide are classified as peroxidases.

<img class=" size-full wp-image-1964 aligncenter" src="http://www.bioadvisers.com/wp-content/uploads/2020/11/Hydrogen-Peroxide.png&quot; alt="Hydrogen Peroxide" width="609" height="373" srcset="http://www.

A Common test of Hydrogen Peroxide

 

Hydrogen Peroxide is a peroxide and oxidizing agent with disinfectant, antiviral and anti-bacterial activities. Upon rinsing and gargling or topical application, hydrogen peroxide exerts its oxidizing activity and produces free radicals which leads to oxidative damage to proteins and membrane lipids. This may inactivate and destroy pathogens and may prevent spreading of infection.

 

Hydrogen peroxide is unstable and slowly decomposes in the presence of light. Because of its instability, hydrogen peroxide is typically stored with a stabilizer in a weakly acidic solution in a dark coloured bottle. Hydrogen peroxide is found in biological systems including the human body. Enzymes that use or decompose hydrogen peroxide are classified as peroxidases.

<img class=" size-full wp-image-1964 aligncenter" src="http://www.bioadvisers.com/wp-content/uploads/2020/11/Hydrogen-Peroxide.png&quot; alt="Hydrogen Peroxide" width="609" height="373" srcset="http://www.

Monoamine Oxidase and Depression

 

Monoamine oxidase is a type of enzyme that helps neurons fire throughout your body. It’s formed in your liver and cleans up neurotransmitters in your brain once they’ve done their jobs. Besides neurotransmitters, monoamine oxidase cleans out tyramine, a chemical that helps regulate blood pressure.

 

Monoamine oxidase inhibitors (also called MAO inhibitors or MAOIs) block the actions of monoamine oxidase enzymes. Monoamine oxidase enzymes are responsible for breaking down neurotransmitters such as dopamine, norepinephrine, and serotonin in the brain.

 

<img class="alignnone wp-image-1959 aligncenter" src="http://www.bioadvisers.com/wp-content/uploads/2020/09/Fig1:Physiological-functions-of-MAO-and-MAOI.jpg&quot; alt="" width="499" height="386" srcset="http://www.bioadvisers.com/wp-content/uploads/2020/09/Fig1:Physiological-functions-of-MAO-and-MAOI.jpg 850w, http://www.bioadvisers.com/wp-content/uploads/2020/09/Fig1:Ph

New hydrogel enables large-scale proliferation of T cells

 

Spanish researchers have recently designed a new type of hydrogel (hydrogel) that can cultivate T lymphocytes. These hydrogels can mimic lymph nodes and allow T cells to expand, thus bringing a high cell proliferation rate. This result was published in the online edition of Biomaterials.

 

There have been many new developments in the field of immunotherapy in recent years, such as the transformation of T cells for adoptive cell therapy. These results are expected to introduce more effective anti-cancer strategies, but there are also many limitations. For example, T cells are still challenging in manufacturing and manipulation, that is, it is difficult for people to produce a large number of therapeutic T cells in a short time.

 

Spanish researchers have recently designed a new type of hydrogel that can culture T lymphocytes on a large scale. These hydrogels can mimic lymph nodes and allow T cells to expand, thus bringing a high cel

New hydrogel enables large-scale proliferation of T cells

 

Spanish researchers have recently designed a new type of hydrogel (hydrogel) that can cultivate T lymphocytes. These hydrogels can mimic lymph nodes and allow T cells to expand, thus bringing a high cell proliferation rate. This result was published in the online edition of Biomaterials.

 

There have been many new developments in the field of immunotherapy in recent years, such as the transformation of T cells for adoptive cell therapy. These results are expected to introduce more effective anti-cancer strategies, but there are also many limitations. For example, T cells are still challenging in manufacturing and manipulation, that is, it is difficult for people to produce a large number of therapeutic T cells in a short time.

 

Spanish researchers have recently designed a new type of hydrogel that can culture T lymphocytes on a large scale. These hydrogels can mimic lymph nodes and allow T cells to expand, thus bringing a high cel

Understand the test of Lactate dehydrogenase helps a lot

 

Lactate dehydrogenase (LDH) is a protein that helps produce energy in the body. An LDH test measures the amount of LDH in the blood. LDH is expressed extensively in body tissues, such as blood cells and heart muscle. Because it is released during tissue damage, it is a marker of common injuries and disease such as heart failure.

 

You may need this test if your healthcare provider suspects you have had a heart attack or may have tissue damage or disease. If you’ve already been diagnosed with a specific disease, you may have this test so your provider can watch your condition and see whether your treatment is working. If the blood sample is contaminated or your red blood cells are broken, your LDH will be higher.

 

<img class="size-full wp-image-1951 aligncenter" src="http://www.bioadvisers.com/wp-content/uploads/2020/08/Lactate-dehydrogenase.jpg&quot; alt="" width="678" height="307" srcset="http://www.bioadvisers.com/wp-content/u